The electrophoresed antigen mixture in agarose gel allows the separation of different antigens along the gel slide, and then the lateral diffusion of an antibody in the gel occurs resulting in precipitate formation. Counter Current Immunoelectrophoresis is a modification of immunoelectrophoresis in which antigen and antibody move in opposite directions and form precipitates in the area where they meet in concentrations of optimal proportions. The technique is similar to the Ouchterlony method, the only difference being that the antigen movement is facilitated by electrophoresis. Objectives Countercurrent immunoelectrophoresis is mostly carried out with one or more of the following objectives: To rapidly check any antisera for the presence and specificity of antibodies for a particular antigen. The test is performed on a glass slide in agarose gel of high electro-endosmotic flow. A pair of wells is punched out where one well is filled with antigen and the other with the antibody.
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Counter-current immunoelectrophoresis for the diagnosis of pneumococcal chest infection.
Counter Current Immunoelectrophoresis